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Academy of Mathematics and Systems Science, CAS
Colloquia & Seminars

Speaker:

Dr.Xingqi Chen,Department of Cell and Molecular Biology, Karolinska Institutet, Sweden

Inviter: 张世华 研究员
Title:
Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity
Time & Venue:
2018.11.9 10:00 S705
Abstract:
Here we introduce Protein-indexed Assay of Transposase Accessible Chromatin with sequencing (Pi-ATAC) that combines single-cell chromatin and proteomic profiling. In conjunction with DNA transposition, the levels of multiple cell surface or intracellular protein epitopes are recorded by index flow cytometry and positions in arrayed microwells, and then subject to molecular barcoding for subsequent pooled analysis. Pi-ATAC simultaneously identifies the epigenomic and proteomic heterogeneity in individual cells. Pi-ATAC reveals a casual link between transcription factor abundance and DNA motif access, and deconvolute cell types and states in the tumor microenvironment in vivo. We identify a dominant role for hypoxia, marked by HIF1 protein, in the tumor microvenvironment for shaping the regulome in a subset of epithelial tumor cells.

Affiliation: 陈兴启, 瑞典卡罗林斯卡医学院,课题组组长,研究员。 本科和硕士就读于西北农林科技大学,(2001-2008), 与2014年瑞典卡罗林斯卡医学院获得博士学位。 博士毕业后加入斯坦福大学医学院Howard Chang 课题组进行博士后研究工作 (2015,3-2018.8)。与2018年9月在瑞典卡罗林斯卡医学院担任PI并组建自己研究团队。陈兴启博士长期从事表观遗传学和功能基因组学相关单细胞技术的开发,在博士和博后期间以第一作者和共同作者发表论文多篇。
 

 

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